Fig. 4.

Effects of DIM (0.01-10 µM) on the ischemia-induced (6 h) LDH release (panel a) and caspase-3 activity (panel b) in primary cultures of mouse hippocampal cells at 2 DIV. Hippocampal cells were simultaneously treated with DIM or vehicle (0.1% DMSO) and ischemia for 6 h. The results are presented as the percentage of ischemic control. Each bar in the graph represents the mean ± SEM of three independent experiments. The number of repeats for each experiment ranged from 7 to 10. ***p < 0.001 versus normoxic cultures; ^###p < 0.001 versus the cultures exposed to ischemia