Fig. 5.

SNO proteins relevant in heat shock response and hypoxia. a Structures of HYOU1, heat shock proteins (HSP), and PDIA1 with highlighted SNO sites. The color gradient of the highlighted background of Cys symbolizes the R _{[GSNO]:[Control]} ratio. The yellow highlights on the protein structure correspond to the position of the Cys. Protein structures are obtained from SWISS-MODEL⁷¹. b SNO sites (red) of CITED4 (novel site detected by Cys-BOOST) and HIF1α (detected formerly) both located at C-termini and putatively involved in activation of hypoxia-responsive genes. Cys 184 of CITED4 was detected as free. c All nine Cys of NOSIP were identified, four of which were free Cys (green) and five were nitrosylated (red). Source data are provided as a Source Data file