Fig. 1.

PfCINCH (P. falciparum coordinator of nascent cell detachment) is a member of the Plasmodium basal complex. a Schematic of inner membrane complex (IMC) and basal complex formation in schizogony. At 42–44 h post invasion, basal complex formation is nucleated near the apical ends of forming daughter cells at the basal end of the IMC. As segmentation progresses from ~44–46 h, the basal complex resides at the leading edge of membrane formation, while the IMC stretches from the basal complex to the apical pole of the cell. When segmentation is complete at ~46–48 h, the IMC extends from the apical to the basal pole of the cell, while the basal complex resides exclusively at the posterior end of the merozoite. b Confocal microscopy of PfCINCH and PfGAP45 (IMC marker) throughout segmentation. c Airyscan super-resolution microscopy (1.7-fold resolution increase) of PfCINCH and PfMORN1 (basal complex marker) throughout segmentation. d Ortho-slice of PfMORN1 and PfCINCH at the beginning of segmentation. All scale bars 1 µm