Fig. 3.

Segmentation is impaired in PfCINCH (P. falciparum coordinator of nascent cell detachment)-deficient parasites. a Airyscan super-resolution micrographs of merozoite plasma membrane and micronemes, stained with antibodies against P. falciparum merozoite surface protein 1 (PfMSP1) and PfAMA1, respectively, in E64-treated schizonts cultivated with and without anhydrotetracycline (ATc). Following the egress trigger, P. falciparum apical membrane antigen 1 (PfAMA1) is translocated from micronemes to the merozoite membrane (bottom panels). b Confocal micrographs of PfGAP45 (IMC marker) and residual PfCINCH over the course of segmentation in PfCINCH-deficient parasites. PfCINCH is visible in schizonts, but forms relatively larger rings that surround multiple nuclei as merozoites are formed. All scale bars 1 µm