Fig. 3.

Expression of CXCR3B in human melanocytes and their regulation by IFNγ. a Total CXCR3 mRNA (black + white bars) and CXCR3B mRNA (black bars) in healthy and vitiligo primary melanocytes (n = 8) and healthy keratinocytes (n = 5) before and after exposure to IFNγ 50 ng/ml for 24 h. Results are normalized to unstimulated melanocytes from healthy subjects and expressed as mean ± SEM. b Immuno-detection of CXCR3B protein and c its semi-quantification in melanocytes extracted from healthy skin (open circles, n = 6–7) and skin from vitiligo patients (closed circles, n = 7) before and after IFNγ stimulation. d In situ detection of CXCR3B+ melanocytes (MITF+CXCR3B+) in vitiligo non-lesional (NL) skin and their quantification (n = 5) compared to healthy skin (n = 5). Results are shown as individual dot plots with a line at median