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. 2019 May 16;10:2193. doi: 10.1038/s41467-019-10229-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Reduction of endocytosis restores the Ptp4EPtp10D phenotypes. a Confocal images displaying the dorsal trunk (DT) of late-stage 16 (left column) and early-stage 17 (right column) wild-type, Ptp4EPtp10D, Ptp4EPtp10D;rab5² and Ptp4EPtp10D;vps45^JJ−2 embryos stained for the luminal protein Verm (grey). Scale bars, 10 μm. b Plots showing the average number of wild-type (n = 24), Ptp4EPtp10D (n = 66) and Ptp4EPtp10D; rab5² (n = 59) mutant embryos with luminal Dextran-TR at 17.5 h after egg laying (1.5 h prior to the luminal clearance initiation in wild type). The premature luminal clearance phenotype of Ptp4EPtp10D is suppressed in Ptp4EPtp10D;rab5² triple mutant embryos. **P < 0.001. c Plots depicting the average DT (Tr8) diameter in wild-type (n = 38), Ptp4EPtp10D (n = 45), Ptp4EPtp10D;rab5²/+ (n = 28), Ptp4EPtp10D;rab5² (n = 56), Ptp4EPtp10D;vps45^JJ−2 (n = 29), Ptp4EPtp10D;btl>Shi^RM (n = 37), Ptp4EPtp10D;rab11^j2D1 (n = 22), Ptp4EPtp10D;btl>Rab11^S25N (n = 47) and Ptp4EPtp10D;btl>Rab11^Q70L (n = 27) embryos. The DT diameter phenotype observed in Ptp4EPtp10D mutants is significantly restored in Ptp4EPtp10D;rab5², Ptp4EPtp10D;vps45^JJ−2 and Ptp4EPtp10D;btl>Shi^RM embryos. ***P < 0.001; **P < 0.005. Unpaired two tailed t tests were performed in relation to Ptp4EPtp10D mutant data set (b, c). Error bars show s.e.m.