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. 2019 May 16;9:7464. doi: 10.1038/s41598-019-42849-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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secCl protein is expressed in the apical membrane of stellate cells. Immunostaining of adult MTs using anti-secCl (green). Nuclei are labelled with DAPI (blue). (a) secCl is expressed in the stellate cells but not the principal cells of MTs. (b) Immunostaining is not observed in homozygous mutant secCl^G689/G68933 MTs. (c) Immunostaining is restored to stellate cells in homozygous mutant secCl^G6893/G6893 bearing a single copy of a wild-type secCl transgene, p[secCl]. (d) An optical cross-section of MTs stained with anti-secCl showing localization to apical membrane (luminal side of the nucleus). (e) Expression of the transgenic mCherry-tagged CD8 (red), which is a marker of the apical membrane in polarized epithelia. (f) Merge of (d) and (e) showing co-localization of secCl and CD8 (yellow). (g–i) A z-stack rendered in 3D and rotated showing single stellate cell. Colors as in (d–f). Scale bars in (a–c) represent 50 μm; (d–f) 10 μm; (g–i) 5 μm.