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. 2019 May 16;9:7464. doi: 10.1038/s41598-019-42849-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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secCl is required for the tyramine-mediated diuretic response. Fluid secretion assays conducted on tubules with the genotypes: w (secCl(+)), w; secCl^G6893/G6893 (secCl(−)) and w; p[secCl], secCl^G6893/G6893 (secCl(−) rescue). (a) Basal fluid secretion rate (FSR) in Drosophila saline⁴² over 40 minutes. (b) Change in FSR in response to 2.9 μM tyramine or Drosophila saline (mock treatment) normalized to the first 40 minute interval. Red horizontal bar indicates the mean and error bars represent standard error of the mean. *** indicates p < 10⁻⁵. Significance was estimated by ANOVA and pairwise comparisons by Tukey’s HSD.