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. 2019 May 16;9:7464. doi: 10.1038/s41598-019-42849-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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secCl is necessary for the decrease in transepithelial potential in response to tyramine and leucokinin. Responses of the transepithelial potential (TEP) to tyramine. (a–d) Recordings of TEP in isolated Malpighian tubules. ‘*’ indicates penetration of the electrode into the lumen and ‘**’ indicates exit of electrode. The shaded area indicates the period of perfusion with saline or 2.9 μM tyramine as indicated. The beginning of perfusion was marked by a recording artifact. (a) The TEP of control w (secCl(+)) tubules did not respond to perfusion with saline. (b) In response to tyramine, the TEP of control tubules dropped to near zero mV and began to oscillate. (c) Tubules from w; secCl^G6893/G6893 (secCl(−)) flies did not respond to tyramine with a voltage drop. (d) Rescuing the secCl^G689/G68933 with a wild-type transgene (w; p[secCl]/+; secCl^G6893/G6893) restored the voltage drop and oscillations in response to tyramine. (e) P-values indicate the significance of the median change in absolute TEP pre- vs post-treatment (15 seconds after perfusion with saline control, 2.9 μM tyramine or 5 μM leucokinin) by Mann-Whitney U test.