Figure 3.

Hepatic expression of FSP27β strongly increased with the severity of NAFLD whereas CIDEA expression decreased. Wild-type mice fed a control diet (Ctrl D) (n = 4) or MCDD (n = 6) for 2 and 7 weeks. (A, B, C) Hepatic expression of CIDEA, CIDEB, FSP27α and FSP27β was evaluated in Wt, Ctrl D and MCDD mice at the mRNA level (4–6 mice/group). The gene expression was normalized to the mRNA levels of B2M or 36B4. Results are expressed relative to the expression level in controls (means ± SEM) and statistically analyzed using the Mann–Whitney test. (D) Hepatic expression of CIDEA, FSP27 and HSP90 was evaluated in Wt, Ctrl D and MCDD mice at the protein level (2–5 mice/group). *, versus Ctrl D, p < 0.05