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. 2019 May 16;9:7501. doi: 10.1038/s41598-019-43928-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Down regulation of FSP27β resulted in the fractionation of lipid droplets in AML12 hepatocytes. AML12 hepatocytes after control (siCtrl) or FSP27 silencing (si FSP27) were stimulated with oleic acid (0.5 mM) for 16 h (n = 3). (A) FSP27α and FSP27β mRNA expression levels were analyzed by real-time quantitative PCR. The gene expression was normalized to RPLP0 mRNA levels. (B,C) Lipid droplets were stained by Oil-Red O solution for 5 min. (B) Representative pictures are shown. (C) Lipid droplet areas were quantified (in pixels squared) and normalized according to the number of nuclei on the slides. Results are expressed relative to the control (siCtrl) as means ± SEM. Data were statistically analyzed using the Student’s t-test. *, versus siCtrl, p < 0.05.