Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 16;9:7501. doi: 10.1038/s41598-019-43928-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Overexpression of FSP27β enhanced cell death in response to TNFα and palmitic acid in HEPG2 hepatocytes. (A) HEPG2 cells were transfected with pCMV-HA and pCMV-HA-FSP27β as indicated. After 48 h, the human BCL2, mouse FSP27 and human CIDEC mRNA expression levels were analyzed by real-time quantitative PCR. The gene expression values were normalized to RPLP0 mRNA levels (n = 4). (B,C) After overexpression of FSP27β with pCMV-HA-FSP27β transfection in HEPG2 cells, (B) cell death (flow cytometry)(n = 4) were evaluated in the basal state and in response to TNFα (20 ng/ml) and actinomycin D (0.1 µg/ml) with TNFα (20 ng/ml) for 16 h and (C) cell viability (MTT assay)(n = 3), in response to palmitic acid (1 mM) for 16 h. Results relative to the control (pCMV-HA) are expressed as means ± SEM. Data were statistically analyzed using the Student’s t-test. *, versus pCMV-HA p < 0.05.