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. 2019 May 16;9:7488. doi: 10.1038/s41598-019-43960-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thin layer chromatography (TLC) showing organic fraction with antimicrobial activity from Streptomyces sp. So13.3 crude extract against MRSA and S. aureus ATCC 25923. TLC was revealed by spraying with ninhydrin reagent or UV light exposition and TLC‒bioautography assay on MRSA and S. aureus ATCC 25923 is shown. Active fraction of the crude extract in the TLC is shown with a circle selection (retardation factor (R_f) = 0.78).