. 2019 Apr 9;25(3):787–797. doi: 10.1007/s12298-019-00669-1

Table 2.

Comparison of amino acids content in the different hairy root transformed lines from leaf and stem

Compounds	Control root	Leaf			Stem
Compounds	Control root	(Line 1)	(Line 2)		(Line 1)	(Line 2)	(Line 3)
Asparagic acid	7.1 ± 0.33	10.9 ± 0.31^a	12.6 ± 0.43^a	10.3 ± 0.30^a	16.1 ± 0.51^a	14.7 ± 0.44^a	12.5 ± 0.35^a
Threonine	4.0 ± 0.37	6.3 ± 0.76^a	6.8 ± 0.67^a	5.4 ± 0.56^a	8.4 ± 1.11^a	7.8 ± 0.87^a	6.9 ± 0.57^a
Serine	4.6 ± 0.31	6.0 ± 0.80^a	7.6 ± 0.69^a	6.2 ± 0.67^a	8.6 ± 0.80^a	7.8 ± 0.78^a	6.8 ± 0.62^a
Glutamic acid	31.8 ± 2.42	47.6 ± 3.86^a	25.5 ± 2.34^a	19.4 ± 2.97^a	38.4 ± 2.22^a	32.0 ± 2.51^b	45.1 ± 3.94^a
Proline	0.8 ± 0.12	3.0 ± 0.14^a	3.9 ± 0.21^a	2.3 ±0.19^a	5.1 ± 0.29^a	9.9 ±0.21^a	3.9 ± 0.28^a
Glycine	4.4 ± 0.27	6.0 ± 0.39^a	7.1 ± 0.44^a	5.5 ± 0.48^a	8.2 ± 0.56^a	7.8 ± 0.59^a	6.5 ± 0.79^a
Alanine	5.1 ± 0.44	7.7 ± 0.64^a	11.7 ± 0.54^a	9.5 ± 0.57^a	14.2 ± 0.47^a	11.4 ± 0.48^a	8.8 ± 0.38^a
Valine	4.5 ± 0.28	6.5 ± 0.48^a	7.8 ± 0.53^a	6.4 ± 0.47^a	8.6 ± 0.36^a	8.0 ± 0.41^a	7.1 ± 0.51^a
Isoleucine	3.2 ± 0.27	4.6 ± 0.36^a	5.5 ± 0.45^a	4.7 ± 0.73^a	6.4 ± 0.83^a	5.9 ± 0.79^a	5.2 ± 0.48^a
Leucine	5.7 ± 0.56	8.6 ± 0.54^a	10.5 ± 0.67^a	8.8 ± 0.76^a	12.1 ±0.79^a	11.3 ± 0.67^a	9.8 ± 0.57^a
Tyrosine	2.4 ± 0.26	2.2 ± 0.35^b	2.5 ± 0.47^b	1.4 ± 0.56^a	2.4 ±0.29^b	2.7 ± 0.32^b	2.3 ± 0.38^b
Phenylalanine	3.1 ± 0.36	4.4 ± 0.37^a	5.6 ± 0.41^a	4.5 ± 0.37^a	6.4 ± 0.35^a	6.1 ± 0.29^a	5.0 ± 0.34^a
Histidine	4.6 ± 0.46	5.9 ± 0.34^a	6.4 ± 0.35^a	5.3 ± 0.43^a	7.5 ± 0.45^a	7.0 ± 0.48^a	6.2 ± 0.39^a
Lysine	5.4 ± 0.35	8.4 ± 0.28^a	10.0 ± 0.86^a	8.6 ± 0.53^a	11.8 ± 0.34^a	10.9 ± 0.37^a	9.6 ± 0.43^a
Arginine	3.3 ± 0.39	5.3 ± 0.42^a	6.8 ± 0.43^a	5.4 ± 0.28^a	7.8 ± 0.34^a	7.1 ± 0.37^a	6.5 ± 0.32^a
Cystine	ND	0.8 ± 0.13	1.3 ± 0.09^a	1.1 ± 0.12^a	1.3 ± 0.23	1.7 ± 0.21^a	0.8 ± 0.08^a
Methionine	ND	0.9 ± 0.21	1.6 ± 0.11^a	1.6 ± 0.11^a	1.7 ± 0.33	2.1 ± 0.20^a	1.1 ± 0.09^a
Total amino acids	89.9 ± 6.83	135.2 ± 10.37^a	133.1 ± 9.69^a	106.3 ± 10.1^a	165.0 ± 10.27^a	154.2 ± 9.99^a	144.0 ± 10.52^a

Data represented mean ± SD, n = 5

ND not detected. The measurement unit used is g per 1000 g analytical DM

a letter in column are significant at the P ≤ 0.05

b letter in column are not significant at the P ≤ 0.05