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. 2019 May 16;9:7485. doi: 10.1038/s41598-019-44011-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram of the IC-qPCR in the quantification of PT modification frequency. (i) Left (Reaction A): DNA sample was treated with iodine, Right (Reaction B): DNA sample was treated with H₂O instead of iodine. (ii) The treated DNAs were quantified by real-time PCR analysis, (iii) PT modification frequency of each PT site was calculated according to the formula.