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. 2019 May 16;9:7485. doi: 10.1038/s41598-019-44011-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amplification plots and standard curves of four IC-qPCR assays using E. coli B7A DNA calibrators ranged from 5 to 1 × 10⁶ copies per reaction. (a) 607710 assay, (b) 1818096 assay, (c) 3026955 assay, (d) 4120753 assays. Each reaction was performed in quintuplicates.