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. 2019 May 16;9:7485. doi: 10.1038/s41598-019-44011-x

Table 1.

Sensitivity of IC-PCR assays for the dilution series of E. coli B7A DNA.

Array Copies/reaction Number of positive Mean Ct values Amount copy number RSD (%) Bias (%)
607710 100 15/15 30.28 99.05 1.43 −0.95
10 15/15 33.33 12.13 12.99 21.30
5 8/15 / / / /
2 0/15 NA NA NA NA
100 15/15 31.16 94.24 7.40 −5.76
1818096 10 15/15 34.29 11.28 19.27 12.80
5 3/15 / / / /
2 0/15 NA NA NA NA
100 15/15 30.73 87.57 5.85 12.43
3026955 10 15/15 33.82 11.91 3.29 11.91
5 15/15 34.73 6.62 9.91 32.37
2 0/15 NA NA NA NA
100 15/15 29.45 109.72 3.93 9.72
4120753 10 15/15 32.84 11.37 12.48 13.70
5 15/15 34.07 4.99 23.46 −0.20
2 2/15 / / / /

“/” Means no enough data for further analysis, “NA” means no amplification.