Fig. 1.

Increased temporal lobe mRNA expression of IDE and RCAN1 in i.c. STZ-treated rats. Long Evans rats were administered single i.c. injections of STZ or vehicle at 4 weeks of age (N = 6/group). The rats were sacrificed 4 weeks later to measure temporal lobe mRNA expression of (A) IDE and (B) RCAN1 by qRT-PCR analysis, with results normalized to HPRT measured simultaneously in the same samples. Graphs depict box plots with mean levels of expression (horizontal bars), 95% confidence interval limits (upper and lower boundaries of the boxes) and ranges (upper and lower stems). Student t tests were used for intergroup comparisons. Abbreviations: IDE, Insulin-degrading enzyme; RCAN1, regulator of calcineurin 1; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; HPRT, hypoxanthine phosphoribosyltransferase; STZ, streptozotocin.