Fig. 2.

Increased temporal lobe protein expression of IDE and RCAN1 in i.c. STZ-treated rats. Long Evans rats were given single i.c. injections of STZ or vehicle at 4 weeks of age (N = 6/group). Four weeks later, the rats were sacrificed to measure temporal lobe protein expression of (A) IDE and (B) RCAN1 by duplex direct-binding ELISAs with results normalized to RPLPO measured sequentially in the same samples. Graphs depict box plots with mean levels of expression (horizontal bars), 95% confidence interval limits (upper and lower boundaries of the boxes), and ranges (upper and lower stems). Student t tests were used for intergroup comparisons. Abbreviations: IDE, Insulin-degrading enzyme; RCAN1, regulator of calcineurin 1; STZ, streptozotocin; ELISA, enzyme-linked immunosorbent assays; RPLPO, ribosomal protein.