Fig. 5.

Effects of AD Braak stage and ApoE genotype on RCAN1 gene expression in human postmortem brains. Fresh frozen postmortem human frontal lobe tissue samples from cases with Braak stage (B) 0-2 (normal aging), B3-4 (moderate AD), or B5-6 (severe AD) were used to measure RCAN1 mRNA expression by qRT-PCR analysis with results normalized to HPRT mRNA measured simultaneously in the same samples. Graphs depict box plots with mean levels of expression (horizontal bars), 95% confidence interval limits (upper and lower boundaries of the boxes), and ranges (upper and lower stems). Statistical comparisons assessed changes in RCAN-1 mRNA expression in relation to: (A) AD severity in cases with ApoE ε3/ε3 genotype, (B) ApoE genotype in cases with moderate AD (B3-4), and (C) ApoE genotype in cases with severe AD. One-way ANOVA with post hoc repeated measures Tukey tests were used for intergroup comparisons. Abbreviations: AD, Alzheimer's disease; ANOVA, analysis of variance; ApoE, apolipoprotein E; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; RCAN1, regulator of calcineurin 1; HPRT, hypoxanthine phosphoribosyltransferase.