Table 1.
Primer pairs used for qRT-PCR analysis of human and rat IDE and RCAN genes
| Primers | Direction | Sequence | Position | Amplicon |
|---|---|---|---|---|
| Human | ||||
| IDE | Forward | TACCTCCGCTTGCTGATGAC | 2110 | 106 |
| IDE | Reverse | GGAGCTGAGGTATGAAGGCC | 2215 | |
| RCAN1 | Forward | AGGCTCCAGCTGCATAAGAC | 258 | 111 |
| RCAN1 | Reverse | CTGCTTGTCTGGATTTGGCG | 368 | |
| Rat | ||||
| IDE | Forward | CTGTGCCCCTTGTTTGATGC | 523 | 139 |
| IDE | Reverse | TGAAGGGGTGCTTGGGATTC | 661 | |
| RCAN1-2-3 | Forward | AACTTCAGCAACCCCCTGTC | 231 | 80 |
| RCAN1-2-3 | Reverse | AGTTTCATCTCCTTCCCCAGG | 310 |
Abbreviations: IDE, Insulin-degrading enzyme; RCAN1, regulator of calcineurin 1; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.