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. 2019 May 14;18:100645. doi: 10.1016/j.bbrep.2019.100645

Fig. 2.

Fig. 2

EGFP-2A-Eluc transgenic mouse and rats express GFP fluorescence and ELuc luciferase in all organs. (A) GFP fluorescence and bioluminescence images of intraperitoneal organs taken from transgenic mouse and rats. All organs expressed both GFP and ELuc signals (Experiments were performed in two to three times independent experiments, each with similar results). (B) Representative images of GFP fluorescence and ELuc bioluminescence in transgenic mouse and rat organs (B; brain, H; heart, L; liver, K; kidney, T; testis) (mouse n = 3/rats n = 3). (C) Representative pictures of transgenic mouse and rat tissue sections stained with GFP or ELuc antibody respectively (liver, kidney, heart, muscle) (mouse n = 3/rats n = 3). Scale bars: 100μm. (D) Exogenous GFP and ELuc expression in transgenic mouse and rat organs were confirmed by western blotting using α-GFP and α-ELuc antibody. α-β-tubulin was used as a loading control. (n = 3).