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. 2019 May 14;18:100645. doi: 10.1016/j.bbrep.2019.100645

Fig. 3.

Fig. 3

Differential expression pattern of GFP fluorescence and ELuc luciferase in EGFP-2A-ELuc transgenic mouse and rats. (A) Bright field (left) and GFP fluorescence (right) images of isolated ADSCs from transgenic mice (left) or rats (right). All cells indicated GFP fluorescence. Scale bars: 100 μm. (B) FCM analysis of GFP expressing ADSCs established from transgenic mouse (gray column) and rat (black column). Wild-type rat ADSCs used as negative control (white column). Each bar represents the mean ± S.D. (n = 4). (C) Luciferase signals detected from living and lysed ADSCs derived from transgenic mice (gray column) and rats (black column) respectively. Wild-type rat ADSCs used as negative control (white column). Each bar represents the mean ± S.D. (n = 11–14 in each group). (D) Immunofluosrescence analysis of transgenic mouse and rat ADSCs using α-GFP (green) and α-ELuc (red) antibodies showed the expression of exogenous both GFP and ELuc in individual cells. The cells were counterstained with DAPI (blue). Scale bars: 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)