Fig. 4.

Effects of MagH₂ on negative geotaxis. Shown are fly lines under-expressing mitochondrial Prxs globally (Da-DM), in motor neurons (D42-DM) and in the midgut (NP1-DM), as well as y w control. All flies tested for geotaxis were one week old, or at the age when their mortality did not exceed 10%. Flies were fed hydrogen-producing food starting from day 1–2 after eclosion, or 5–6 days prior to the geotaxis experiments. Assays were performed as indicated in Materials and Methods and geotaxis was expressed as a number of climbers/jumpers to the total number of flies. The activity of approximately 50 flies for each fly line was measured. Analysis was conducted in triplicate for each of two biological replicates. Shown are means ± SEM (n = 6). Asterisks denote statistically significant differences (*P < 0.05).