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. 2019 May 14;5(5):e01679. doi: 10.1016/j.heliyon.2019.e01679

Table 1.

Life spans of flies under different treatments. A and B: The experiments were conducted as described in the Fig. 1 (A) and Fig. 2 (B) legends. Values for median age obtained in two independent experiments are listed in columns 1 and 3. Columns 2 and 4 indicate the percentage of median age changes between experimental flies and flies maintained on a regular food (Control). Statistically significant differences between control and flies treated with MagH2, determined by the log-rank test (*P < 0.05), are indicated by asterisks. C: The experiments were conducted as described in the Fig. 3 legend. The age of the flies at 10% of mortality and the “smurf” phenotype are shown in columns 1 and 3. Values are obtained in two independent experiments. Columns 2 and 4 show the percentage change between flies fed MagH2 and flies fed heated MagH2 solutions that served as a hydrogen-free control. Statistically significant differences between flies fed MagH2 and flies fed heated MagH2 solutions, determined by two-way ANOVA (*P < 0.05), are indicated by asterisks and bold. There were no statistically significant differences between mortality and the development of the “smurf” phenotype.

A
 Males
 Females
Treatment Median age (days)
1		 % vs. Control
2		 Median age (days)
3		 % vs. Control
4
Control 59; 56 54; 51
MagH2 68; 64 115*; 114* 67; 65 124*; 127*
MagH2 heat 58.5; 57 99; 102 57; 52 106; 102
B
 Males
 Females
Treatment Median age (days)
1		 % vs. Control
2		 Median age (days)
3		 % vs. Control
4
Control 12; 8 12; 13
MagH2 0.1 16; 11 133*; 137.5*
MagH2 0.1 heat 12; 8.5 100; 106
MagH2 0.3 11.5; 10 96; 125 15; 15 125*; 115*
MagH2 0.3 heat 11; 10 92; 125* 12; 12 100; 92
MagH2 1 11; 13 92; 100
MagH2 1 heat 12; 11 100; 85
C
 Males
 Females
Treatment Age (days) at 10% mortality/10%”smurf”
1		 % MagH2 0.1 vs. MagH2 0.1 heat
2		 Age (days) at 10% mortality/10% ”smurf”
3		 % MagH2 0.3 vs. MagH2 0.3 heat
4
MagH2 0.1 27; 31/32; 31 142*; 129*/168*; 124*
MagH2 0.1 heat 19; 24/19; 25
MagH2 0.3 34; 33/33; 32 261*; 220*/236*; 200*
MagH2 0.3 heat 13; 15/14; 16