Figure 4. Expression of Notch signaling-related molecules (Hes1 and Hes5) and cellular proliferation of purified CD4⁺ T cells in response to DAPT treatment.

Purified CD4⁺ T cells from 15 GC patients were stimulated with DMSO or DAPT for 24 h. Both (A) Hes1 and (B) Hes5 mRNA levels were significantly down-regulated in response to DAPT stimulation. (C) Hes1 and Hes5 protein levels were also remarkably reduced in response to DAPT stimulation by Western blot tests. (D) Cellular proliferation was measured by cell counting kit-8, and there was no significant difference on cellular proliferation between DMSO and DAPT treatment. Columns presented as means, and bars presented as standard deviations. Paired t tests were used for comparisons.