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. 2019 May 14;39(5):BSR20181245. doi: 10.1042/BSR20181245

Figure 4. The knockdown of BRD4 promoted I-BET151-induced inhibition of osteoclast differentiation.

Figure 4

(A) Representative images of TARCP staining presenting osteoclast formation after RANKL treatment in the presence or absence of I-BET151 in RAW 264.7 cells with or without siBRD4 transfection. (B) The statistical analysis of images from (A) showing the percentage of multinucleated TRACP positive cells. (C) qPCR measurement of osteoclast-specific genes TRACP, MMP-9, Ctsk, and C-Src, from the samples prepared as in (A). Data were normalized to the control. (D) The levels of inflammatory cytokines TNF-α, IL-1β, and IL-6 in the culture medium were detected using ELISA. The result was representative of three independent experiments. Error bars represented mean ± S.D. P-values were determined by the one-way ANOVA followed by Tukey’s post hoc test; ***P<0.001, **P<0.01, *P<0.05.