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. 2019 May 17;39(5):BSR20190357. doi: 10.1042/BSR20190357

Figure 2. CARD kills gastric cancer cell lines.

Figure 2

(A) The inhibition rates of the human gastric cancer cell line treated with increasing concentrations of CARD in 24 or 48 h; (B) the apoptosis ratios were determined by Annexin V-FITC/PI staining; (C) microscopy was used to observe the cell morphology; (D) analysis of the dead and live cell by LIVE/DEAD™ Viability/Cytotoxicity Kit; (E) Western blot was used to measure Bcl-2, Bax, and Caspase-3 protein expression. Three such experiments were quantified by measuring the intensity of apoptosis-related proteins relative to the GAPDH (loading) control. Data represent the mean ± SD of three independent experiments. *P<0.05.