Table I.
Gene symbol | Primer sequence (5′-3′) |
---|---|
LC3 | F: CGGAGCTTTGAACAAAGAGTG |
R: TCTCTCACTCTCGTACACTTC | |
Beclin-1 | F: AGCTCAGTACCAGCGGGAGT |
R: TGGAAGGTGGCATTGAAGAC | |
Atg5 | F: TGTGCTTCGAGATGTGTGGTT |
R: GTCAAATAGCTGACTCTTGGCAA | |
c-Fos | F: CGGGTTTCAACGCCGACTA |
R: TTGGCACTAGAGACGGACAGA | |
NFATc1 | F: GGAGAGTCCGAGAATCGAGAT |
R: TTGCAGCTAGGAAGTACGTCT | |
CatK | F: CTCGGCGTTTAATTTGGGAGA |
R: TCGAGAGGGAGGTATTCTGAGT | |
TRAP | F: CACTCCCACCCTGAGATTTGT |
R: CCCCAGAGACATGATGAAGTCA | |
GAPDH | F: GACTTCAACAGCAACTCCCAC |
R: TCCACCACCCTGTTGCTGTA |
F, forward; R, reverse; LC3, microtubule-associated protein 1 light chain3; NFATc1, nuclear factor of activated T cells 1; CatK, cathepsin K; TRAP, tartrate-resistant acid phosphatase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.