Table I.

Primer sequences of the glomerulosclerosis associated proteins.

Gene	Primer sequence
TGF-β1	F: 5′-ATGAACCGACCCTTCCTGCT-3′
	R: 5′-TGTGTCCAGGCTCCAAATGT-3′
Smad7	F: 5′-CTCTTGCGAACATTACGGCT-3′
	R: 5′-CGAGATCAAGGTCGACCTGC-3′
GADPH	F: 5′-CTACCCACGGCAAGTTCAAT-3′
	R: 5′-GGATGCAGGGATGATGTTCT-3′
Smad2/3	F: 5′-GGAAAGGGTTGCCACATGTT-3′
	R: 5′-AGAATCTCCGTGTGCCGAGG-3′
Smad4	F: 5′-ACGGCCATCTTCAGCACCAC-3′
	R: 5′-AGAATGCACAATCGCCGGAG-3′
ZO-1	F: 5′-GGCATTATTCGCCTTCATAC-3′
	R: 5′-GGAACACAACAATCGGATAC-3′
GSK3β	F: 5′-CTTCAGGACAAGCGATTTA-3′
	R: 5′-CCAGCACCAGGTTAAGGTAG-3′
Nephrin	F: 5′-CCCTCCGGGACCCTACTG-3′
	R: 5′-TCTGGGAGGATGGGATTGG-3′
P-Cadherin	F: 5′-AAGTGCTGCAGCCAAAGACAGA-3′
	R: 5′-AGGTAGACCCACCTCAATCATCCTC-3′
SMA	F: 5′-CAGCTATTGCCGTTCCAATTGA-3′
	R: 5′-CCAGGGCTTCATCATTGCA-3′
FSP	F: 5′-ATACTCAGGCAACGAGGGTG-3′
	R: 5′-CTTCCGGGGCTCCTTATC-3′
TGF-βIR	F: 5′-ACCATTTGGAGCCAGAACAC-3′
	R: 5′-GGTCTGAAGAGCTGAGCCTG-3′
TGF-βIIR	F: 5′-CGAGCTCGGTGGAAGGTCTCATTTTATTG-3′
	R: 5′-CCCAAGCTTGGGATGTAAAAGACAAACAATG-3′
β-catenin	F: 5′-GCTGACCAAAACTGCTAAATGACGA-3′
	R: 5′-TGTAGGGTCCCAGCGGTACAA-3′

ZO-1, zona occludens protein 1; Smad, mothers against decapentaplegic homolog 9; GSK, glycogen synthase kinase; TGF-βR, tumor growth factor β receptor; SMA, smooth muscle actin; FSP, fibroblast-specific protein.