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. 2019 Apr 25;19(6):5335–5344. doi: 10.3892/mmr.2019.10197

Figure 2.

Figure 2.

miR-199a-5p overexpression increases OGD/R-induced cytotoxicity in H9c2 cells. (A) miR-199a-5p expression in H9c2 cells transfected with miR-199a-5p mimic or SC (200 nM) under normoxia for 24 h, as determined by reverse transcription-quantitative PCR analysis. ***P<0.001 vs. control. (B) MTS and (C) LDH assays of control and OGD/R-treated H9c2 cells transfected with miR-199a-5p mimic or control (n=3). *P<0.05. (D) Expression of HIF-1α, GSK3β and p-GSK3β (Ser9) in OGD/R-treated H9c2 cells transfected with miR-199a-5p mimic or control, as determined by western blotting. Protein expression levels of HIF-1α and the relative p-GSK3β/GSK3β protein ratio were quantified using ImageJ software and normalized to β-actin. *P<0.05, **P<0.01 vs. SC. Data are presented as the mean ± standard deviation. Each experiment was repeated three times. miR-199a-5p, microRNA-199a-5p; SC/control, scrambled control; OGD/R, oxygen-glucose deprivation and reperfusion; LDH, lactate dehydrogenase; HIF-1α, hypoxia-inducible factor-1α; p-, phosphorylated; GSK3β, glycogen synthase kinase 3β.