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. 2019 Apr 30;19(6):5321–5334. doi: 10.3892/mmr.2019.10206

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Elevated HP reduced viability and induced apoptosis in RGCs in vitro. These effects were observed using a Cell Counting K-8 assay and by Annexin V FITC/PI flow cytometry, respectively. (A) Annexin V FITC/PI flow cytometry results at different HP values (0, 20, 40, 60 and 80 mmHg). (B) Quantification of cell viability rates. (C) Quantification of cell apoptosis rates. Data are presented as the mean ± standard deviation (n=3). **P<0.01 vs. the 0 mmHg group. HP, hydrostatic pressure; RGC, retinal ganglion cell; PI, propidium iodide; FITC, fluorescein isothiocyanate; UL, upper left; UR, upper right; LL, lower left; LR, lower right.