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. 2019 Apr 30;19(6):5321–5334. doi: 10.3892/mmr.2019.10206

Figure 4.

Figure 4.

Influence of elevated HP on mRNA and protein expression levels in pressure-treated RGCs. (A) NGF, AKT, ASK1, FoxO1 and CREB protein expression levels of RGCs were detected by western blotting. (B) Quantified protein expression of ASK1, FoxO1 and CREB at 20, 40, 60 and 80 mmHg compared with 0 mmHg. (C) Quantified mRNA expression levels of ASK1, FoxO1 and CREB at 20, 40, 60 and 80 mmHg compared with 0 mmHg. To compare the NGF/ASK1/FoxO1 and NGF/AKT/CREB pathways, the expression levels at 0 mmHg were defined as the baseline; the data were calculated by subtracting the protein and mRNA expression levels at 0 mmHg from the protein and mRNA expression levels at 20, 40, 60 and 80 mmHg. (D) Quantified protein expression levels of NGF, AKT, ASK1, FoxO1 and CREB. (E) Quantified mRNA expression levels of NGF, AKT, ASK1, FoxO1 and CREB. Data are presented as the mean ± standard deviation (n=3). #P<0.01 vs. CREB at the same level of HP. *P<0.05 and **P<0.01 vs. the 0 mmHg group. HP, hydrostatic pressure; RGC, retinal ganglion cell; NGF, nerve growth factor; AKT, protein kinase B; ASK1, apoptosis signal-regulating kinase 1; FoxO1, forkhead box O1; CREB, cAMP response element binding protein.