Figure 1.

TSA inhibits the growth of keloid fibroblasts in a time- and dose-dependent manner. (A) Treatment with 1,000 nM TSA altered the morphology of keloid fibroblasts at 24, 48 or 72 h in culture (×100 magnification). Keloid fibroblast phenotypes were examined by phase-contrast microscopy for changes in morphology. (B) The MTT assay indicated that TSA inhibited the cell viability of keloid fibroblasts at concentrations of 250, 500, 1,000, 1,500 nM as observed after 24, 48 or 72 h in culture compared with the control. Results are presented as the mean ± standard deviation of three independent experiments (n=8). One-way analysis of variance with Tukey's post-hoc test was used to compare the groups. *P<0.05, **P<0.01 and ***P<0.001 vs. respective control. TSA, trichostatin A.