Figure 9.

BCL2 is targeted by miR-30a-5p in keloid fibroblasts. (A) Nucleotide sequence of the predicted miR-30a-5p binding site in 3′-UTR of BCL2 mRNA. (B) 293T cells were transfected with luciferase reporter plasmids containing BCL2 3′-UTR and mutant BCL2 3′-UTR or BCL2 3′UTR NC. Luciferase activity was measured 48 h later. Results are presented the mean ± standard deviation of three independent experiments. One-way analysis of variance with Tukey's post-hoc test was used to compare the groups. ***P<0.001. BCL2, B-cell lymphoma 2; miR, microRNA; UTR, untranslated region; NC, negative control; TRAF6, TNF receptor associated factor 6.