Analysis of gene expression in skeletal muscles. (A) RT-sqPCR and (B) densitometry analysis of ESR1, ESR2, CD36, PPARα, ACACA, PLIN2 and FASN in skeletal muscles in three conditions. (C) Western blot and (D) densitometry analyses of CD36, PPARα, PLIN2, FASN, p-ACACA and ACACA in skeletal muscles. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001 vs. sham control group. #P<0.05, ##P<0.01, ###P<0.001 vs. OVX group. RT-sqPCR, reverse transcription-semi-quantitative polymerase chain reaction; PLIN2, perilipin 2; CD36, CD36 molecule; ACACA, acetyl-CoA carboxylase α; FASN, fatty acid synthase; PPARα, peroxisome proliferator activated receptor α; ESR, estrogen receptor; OVX, ovariectomy; E2, 17β-estradiol; p-, phosphorylated; M, marker.