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. 2019 Apr 4;12(5):906–919. doi: 10.1016/j.stemcr.2019.03.002

Figure 3.

Figure 3

CRB1 and CRB2 are Located at the Outer Limiting Membrane in Müller Glial Cells and Photoreceptors of Human iPSC-Derived Retinal Organoids

Immuno-EM staining showing the localization of CRB1 (A–D) and CRB2 (E–H) in DD120 (A, B, E, and F) and DD170 (C, D, G, and H) LUMC0004iCTRL10 human iPSC-derived retinal organoids. CRB1 was lowly and sporadically detected at DD120 but being found subapically of adherens junctions adjacent to photoreceptor inner segments (A) (arrow) and in Müller glial cell apical villi (B) (arrowheads). At DD170, CRB1 was found consistently throughout the outer limiting membrane of human iPSC-derived retinal organoids subapically of adherens junctions of photoreceptors and Müller glial cells (C and D). CRB2 was localized at both DD120 and DD170 subapically of adherens junctions being located in photoreceptor inner segments and apical villi of Müller glial cells. At least two independent samples were analyzed per time point. CC, connecting cilium; AJ, adherens junction; μ, microvilli; IS, inner segment. Scale bar (A–H), 1 μm; insert (B and C), 500 nm.