Figure 3.

AMOT Expression Increases while YAP Is Downregulated during Neuronal Differentiation

(A) Protein levels of AMOT, neuronal marker β-tubulin III (TUJ1), and NSC marker PAX6 during neuronal differentiation from H9 cells. d, day.

(B) Protein levels of YAP and AMOT following treatment for 1 h with DMSO or 1 μM MG-132 at day 6 of neural differentiation.

(C) Protein levels of AMOT, AMOTL1, and AMOTL2 in undifferentiated H9 cells versus H9-derived NSCs.

(D) Subcellular localization of AMOT, F-actin, and YAP in H9-derived NSCs. Filled arrows indicate colocalization. Scale bar, 50 μm. (also applies to E).

(E) Expression of YAP and TUJ1 (left) or AMOT and TUJ1 (right) in Neurog2-T2A-GFP H9 cells, 12 h after induction. Filled arrows indicate GFP⁺ cells, and empty arrows cells with low or absent GFP expression.

See also Figure S2.