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. 2019 May 10;10:503. doi: 10.3389/fpls.2019.00503

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Copyright © 2019 Hamasaki, Kurihara, Kuromori, Kusano, Nagata, Yamamoto, Shimada and Matsui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DEX-inducible KIN10 and KIN11 over-expression plants have decreased polyploidy levels in dark. (A,B) Phenotype of 5-day-old seedlings of vector control (VC), KIN10-dex #1, and KIN10-dex #2 grown on medium containing EtOH (A, upper panel) and 2 mg/ml DEX (A, bottom panel) in the dark. (B) The average of hypocotyl length of vector control (VC), KIN10-dex #1, and KIN10-dex #2. 80–100 seedlings were measured. (C,D) Phenotypes of 5-day-old seedlings of vector control (VC), KIN11-dex #1, and KIN11-dex #2 grown on medium containing EtOH (C, upper panel) and 2 mg/ml DEX (C, bottom panel) in the dark. (D) The average of hypocotyl length of the vector control (VC), KIN11-dex #1, and KIN11-dex #2. 80–100 seedlings were measured. (B,D) Error bars indicate standard deviation. Asterisks indicate significance at P < 0.05 by t-test when compared to VC. (E,F) Relative ratio of each ploidy of dark-grown hypocotyls of VC, KIN10-dex #1, and KIN10-dex #2 grown on medium containing EtOH (E) and 2 mg/ml DEX (F) in the dark. Approximately 20 seedlings were used in ploidy analysis. (G,H) Relative ratio of each ploidy of dark-grown hypocotyls of VC, KIN11-dex #1, and KIN11-dex #2 grown on medium containing EtOH (G) and 2 mg/ml DEX (H) in the dark. Approximately 20 seedlings were used in ploidy analysis. (E–H) Error bars indicate standard error of five biological replicates.