Figure 4.

Defects in CME Show Similar Phenotypes as Loss of Function of Dlg Module or EGFR Overactivation in Cyst Cells

Adult testes of the indicated genotypes in the Gal80^ts background.

(A–D) mCD8-GFP (green; cyst cells), Vasa (red; germline), and TJ (blue; early cyst cell nuclei). Yellow arrowheads indicate mCD8⁺ cyst cell regions.

(E–L) TJ (blue), TUNEL (red; apoptosis), and GFP (green; Rbp4-YFP⁺ spermatocytes or Bam-GFP⁺ spermatogonia). White arrowheads indicate dying spermatogonia. White arrows indicate dying spermatocytes.

(M–P) TJ (red) and dpERK (green). Yellow arrows indicate examples of TJ⁺ and dpERK⁺ cyst cells. Small inset pictures show the dpERK staining.

(Q) Quantification of corrected fluorescent dpERK levels in cyst cell (CC) nuclei.

(R–U) The PIP₂ reporter PLCδ-PH-GFP expressed in cyst cells (green), LamDm0 (red; cyst cells and early germ cells), and TJ (blue).

(V) Quantification of corrected fluorescent PIP₂ levels in cyst cells (CC) via the PLCδ-PH-GFP reporter.

Error bars represent standard error. RNAi activated at 30°C for 2, 3, or 4 days (d). Testes oriented with anterior at left. Image frames, 246 μm (A–H and R–U) and 123 μm (I–P). See also Figures S1 and S2.