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. 2019 Apr 18;12(5):1024–1040. doi: 10.1016/j.stemcr.2019.03.008

Figure 6.

Figure 6

Knockdown of the dPIP5K Kinase sktl in Cyst Cells Can Partially Rescue the EGFR Overactivation Phenotypes and Reduce dpERK Levels

Adult testes of the indicated genotypes in the Gal80ts background.

(A–H) Vasa (red; germline), TJ (blue; early cyst cell nuclei), mCD8-GFP (green; cyst cells), or F-actin stained with phalloidin (green; cyst cells and germline fusome). Small inset pictures show the Vasa staining.

(I–Q) TJ (red) and dpERK (green). Small inset pictures show the dpERK staining.

(R) Quantification of corrected fluorescent dpERK levels in cyst cell (CC) nuclei.

(S) Quantifications of the different phenotypic classes accompanying each genotype, organized in order of phenotypic strength.

Error bars represent standard error. RNAi activated at 30°C for 4 days (d). Testes oriented with anterior at left. Image frames 246 μm (A–H) and 123 μm (I–Q).