Table 3.
Formulation, proximate composition, and essential amino acids of the three diets used in the trial.
| Feed | L1 | L2 | L3 |
|---|---|---|---|
| Feedstuffs (g/kg) | |||
| Fish meal | 180 | 135 | 75 |
| Chironomid meal | 0 | 45 | 90 |
| Soybean meal | 285 | 300 | 300 |
| Wheat meal | 180 | 125 | 125 |
| Hemoglobin | 100 | 100 | 100 |
| Gluten corn | 150 | 190 | 205 |
| Fish oil | 90 | 90 | 90 |
| Vitamin and mineral premix | 15 | 15 | 15 |
| Chemical composition (%) | |||
| Dry matter | 91.19 | 91.25 | 91.08 |
| Crude protein | 45.47 | 45.62 | 45.83 |
| Crude lipid | 13.50 | 13.60 | 13.94 |
| Ash | 5.36 | 5.14 | 4.73 |
| Crude fiber | 2.12 | 2.27 | 2.24 |
| Gross energy (MJ kg) | 16.58 | 16.61 | 16.73 |
| Essential amino acids (g/kg dry matter) | |||
| Arginine (ARG) | 23.5 | 21.0 | 19.7 |
| Histidine (HIS) | 11.5 | 10.0 | 9.5 |
| Isoleucine (ISO) | 24.3 | 22.3 | 21.5 |
| Leucine (LEU) | 34.0 | 33.0 | 30.2 |
| Lysine (LYS) | 28.5 | 27.0 | 26.5 |
| Methionine (MET) | 13.0 | 11.6 | 10.2 |
| Phenylalanine (PHE) | 19.0 | 24.0 | 27.5 |
| Threonine (THR) | 19.7 | 24.0 | 27.3 |
| Tryptophan (TRP) | 4.0 | 4.2 | 4.6 |
| Valine (VAL) | 23.0 | 26.2 | 31.4 |
Feed ingredients were obtained by the Agrarian Corsortium, Sant’Egidio alla Vibrata (TE).