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Design of Experiment (DOE) Variables (First level).
| Independent Variables | Levels | ||
|---|---|---|---|
| −1 | 0 | +1 | |
| Factor 1: Lipid Concentration (mg/mL) | 10 | 20 | 30 |
| Factor 2: Water:Ethanol | 3 | 4 | 5 |
| Factor 3: Drug Concentration (mg/mL) | 1 | 2 | 3 |
| Dependent Variables | Constraints | ||
| Response 1: Size (nm) | Minimum | ||
| Response 2: Entrapment Efficiency (%) | Maximum | ||
| Fixed Variables | Fixed Values | ||
| Stirring Time | 60 min | ||
| Stirring Speed | 1000 rpm | ||
| Cholesterol: Lipid | 1:2 | ||
