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Temperature-dependent coacervation of protamine and multivalent ion mixtures. Turbidity measurements of the (a) protamine/citrate mixture and (b) protamine/TPP mixture at 600 nm with respect to temperature. The total polyelectrolyte concentration (Cp) was fixed to 1% (w/v), and the weight ratio between protamine and multivalent ions was fixed to 6:4. Inset images show reversible phase transitions of the coacervates by temperature cycling.
