Figure 2.

Nocturnal PD effluents from thirty PD patients were subjected to differential centrifugation to isolate an exosome-enriched fraction. Fifteen µg of total proteins from each sample were separated by SDS-PAGE and subjected to immunoblotting with antibodies against AQP1, ALIX and mesothelin. Of note, in 15 randomly chosen samples, there was no band representing the antibodies against the endothelial marked CD31 according to immunodetection. The positive control for CD31 immunoreactivity was a lysate of human omentum.