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. 2019 Apr 10;10(4):294. doi: 10.3390/genes10040294

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Molecular phylogenic tree of the GH6 domain obtained using ORTHOSCOPE analysis (438 nucleotide sites). The GH6 domains of four CesA genes of three tunicates (indicated with arrows) were used as query sequences. The Streptomyces gene (CAB65568) was selected for tree rooting. Because all the non-tunicate metazoans examined do not contain GH6 orthologs, the tree includes only tunicates. It should be noted that tunicate genomes contain not only CesA genes, including the GH6 domain, but also another tunicate-specific GH6 gene.