Table 2.
Summary of proteins incorporated in polyelectrolyte complex micelles.
| Protein | Molecular Weight (kDa) | pI | Appx. Charge 1 | Polyelectrolyte Partners 2 |
|---|---|---|---|---|
| α-Chymotrypsinogen | 25.6 | 8.7 | 4 | PEG-b-p(Asp) [33]; POEGMA-b-q4VP and PAA [140] |
| equine heart cytochrome 3 | 11.8 | 9.6 | 9 | PEG-b-p(Asp-DET) [27]; PEG-b-p(Asp-EDA-Suc) [27] |
| glucose oxidase | 63.3 | 5.1 | −28 | PEG-g-PAA [139] |
| human serum albumin | 66.5 | 6.2 | −11 | PEG-b-PAE-c-API [28] |
| immunoglobulin g (IgG) 3 | 170 | 7.4 | Natively 0.89 | PEG-b-p(Asp-DET) [141] |
| lipase (Humicola lanuginosa) | 31.8 | 5.6 | −7 | P2MVP-b-PEO and PAA [129,142] |
| lysozyme | 14.3 | 7.3 | 8 | PEG-b-p(Asp-EDA-Cit) [26]; POEGMA-b-qP4VP [35] 3; PAA-b-PAAm and PDMAEMA [126,127]; P2MVP-b-PEO and PAA [129]; POEGMA-g-BSA [130]; mPEG-b-p(L-Asp) [133]; PEG-b-p(Asp) [25,132,134] |
| myoglobin 3 | 17.1 | 7.8 | 0 | POEGMA-b-q4VP [35] |
| organophosphate hydrolase | 39.0 | 8.9 | 4 | POEGMA-b-qP4VP and PAA [140] |
| RNase 3 | 16.5 | 8.6 | 6 | POEGMA-b-qP4VP [35] |
| Sprouty 1 | 35 | 8.7 | 8 | POEGMA-g-BSA [130] |
| Fluorescent Proteins | ||||
| EGFP | 26.9 | 6.0 | −7 | P2MVP-b-PEO [137,138] |
| mEGFP | 27.0 | 6.1 | −7 | P2MVP-b-PEO [136] |
| mCherry | 26.7 | 6.5 | −6 | P2MVP-b-PEO [136] |
| mKO2 | 24.5 | 5.9 | −9 | P2MVP-b-PEO [136] |
| mTurquoise2 | 26.9 | 5.8 | −8 | P2MVP-b-PEO, SYFP2, SBFP2, and mTurquoise2 [135]; P2MVP-b-PEO [136] |
| SBFP2 | 26.8 | 6.2 | −7 | P2MVP-b-PEO, SYFP2, SBFP2, and mTurquoise2 [135]; P2MVP-b-PEO [136] |
| SYFP2 | 26.9 | 6.1 | −7 | P2MVP-b-PEO, SYFP2, SBFP2, and mTurquoise2 [135]; P2MVP-b-PEO [136] |
| TagRFP | 26.1 | 7.5 | 0 | P2MVP-b-PEO [136] |
1 The approximate protein charge of the unmodified protein was determined using isolated side chain pKa values and the Henderson-Hasselbalch equation. 2 Abbreviations used here: mPEG-b-p(L-Asp): poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); P2MVP-b-PEO: poly(2-methyl-vinyl-pyridinium)-block-poly(ethylene oxide); PAA: poly(allyl amine); PAA-b-PAAm: poly(acrylic acid)-block-poly(acrylamide); PDMAEMA: poly(N,N-dimethyl aminoethyl methacrylate) oligoethylene glycol methacrylate); PEG-g-PAA: PEG-graft-poly(acrylic acid); PEG-b-PAE-c-API: PEG-block-poly(β-amino ester)-co-1-(3-aminopropyl) imidazole; PEG-b-p(Asp): PEG-block-poly(aspartic acid), with various ⍵-end groups; PEG-b-p(Asp-DET): PEG-block-poly[N-{N′-(2-aminoethyl)-2-aminoethyl}aspartamide]; PEG-b-p(Asp-EDA-Cit): PEG-block-poly[(N′-citraconyl-2-aminoethyl)aspartamide]; PEG-b-p(Asp-EDA-Suc): PEG–block-poly[(N′-succinyl-2-aminoethyl(aspartamide)]; POEGMA-b-qP4VP: poly(oligoethylene glycol methacrylate)-block-poly(4-vinyl N-methyl pyridinium iodide). 3 These proteins were used in a modified form with an unspecified charge.