Table 1.

Characteristics of the different analytical methods developed to perform dexamethasone, hydrochlorothiazide, phenytoin, or spironolactone assay.

	Dexamethasone	Hydrochlorothiazide	Phenytoin	Spironolactone
Mobile Phase	Acetonitrile and water (50:50, v:v)	Methanol and water (20:80, v:v) pH adjusted to 4.5 using acetic acid	Acetonitrile and water (60:40, v:v)	Methanol and water (70:30, v:v)
Flow rate (mL/min)	1.0	1.5	1.0	1.0
Column	Purospher® STAR RP-18 endcapped (5 µm) 150 × 4.6 mm
λ (nm)	238	224	238	238