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. 2019 Apr 8;8(4):329. doi: 10.3390/cells8040329

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Protective action of MALS on myelin sparing in the injured cord. The image shows the protective action of MALS tissue in the epicenter of the injured cord (please see schematic representation). After animal perfusion, spinal cords were dissected, postfixed, and longitudinally sectioned by means of a cryostat. Spinal cord tissue sections were stained for myelin basic protein (MBP, green). The confocal microscope images for the spinal cord of lesioned animals and transplanted with LS or MALS tissue were obtained using the same intensity, pinhole, wavelength, and thickness of the acquisition. Scale bar: 100 µm. The graph reported shows the quantification of fluorescence with reference to MBP staining. Data is expressed as mean of twelve different fields (n = 4 mice; 3 fields/mouse for each condition). Values represent mean ± SD. We determined the statistical differences by means of one-way ANOVA test followed by Bonferroni’s post-test. *** p < 0.001; *p < 0.05 vs. LES; ### p < 0.001 vs. LS.