Figure 3.

Genotyping by HMA assay in the un-injected and injected sterlet embryos. PCR products encompassing sgRNAs target sites were analyzed using a microchip electrophoresis system. (A) Bands from DNA of two injected embryos (sample number 3 and 6) were found to have different size; (B) HMA of two embryos having different bands (sample number 2 and 3). The sample 2 and 3 in Figure 3B were numbered as 3 and 6 in Figure 3A. Heteroduplex bands and multiple short bands are shown in sgRNA+Cas9 RNP injected embryos (crispants; B: sample 2 and 3).